Survival of hypoxic human mesenchymal stem cells is enhanced by a positive feedback loop involving miR-210 and hypoxia-inducible factor 1
Journal of Veterinary Science
;
: 69-76, 2013.
Artigo
em Inglês
| WPRIM
| ID: wpr-219416
ABSTRACT
The use of mesenchymal stem cells (MSCs) has emerged as a potential new treatment for myocardial infarction. However, the poor viability of MSCs after transplantation critically limits the efficacy of this new strategy. The expression of microRNA-210 (miR-210) is induced by hypoxia and is important for cell survival under hypoxic conditions. Hypoxia increases the levels of hypoxia inducible factor-1 (HIF-1) protein and miR-210 in human MSCs (hMSCs). miR-210 positively regulates HIF-1alpha activity. Furthermore, miR-210 expression is also induced by hypoxia through the regulation of HIF-1alpha. To investigate the effect of miR-210 on hMSC survival under hypoxic conditions, survival rates along with signaling related to cell survival were evaluated in hMSCs over-expressing miR-210 or ones that lacked HIF-1alpha expression. Elevated miR-210 expression increased survival rates along with Akt and ERK activity in hMSCs with hypoxia. These data demonstrated that a positive feedback loop involving miR-210 and HIF-1alpha was important for MSC survival under hypoxic conditions.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Oxigênio
/
Consumo de Oxigênio
/
Sobrevivência Celular
/
Regulação da Expressão Gênica
/
Cobalto
/
MicroRNAs
/
RNA Interferente Pequeno
/
Subunidade alfa do Fator 1 Induzível por Hipóxia
/
Células-Tronco Mesenquimais
Limite:
Humanos
Idioma:
Inglês
Revista:
Journal of Veterinary Science
Ano de publicação:
2013
Tipo de documento:
Artigo
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