Molecular characterization of Acanthamoeba isolated from amebic keratitis related to orthokeratology lens overnight wear
The Korean Journal of Parasitology
;
: 313-320, 2006.
Artigo
em Inglês
| WPRIM
| ID: wpr-220307
ABSTRACT
In an effort to characterize, on the molecular scale, the Acanthamoeba initially isolated from the cornea of an amoebic keratitis patient associated with overnight-wear orthokeratology lens in Korea, we conducted mitochondrial DNA restriction fragment length polymorphism, 18S rDNA sequencing, and drug sensitivity analyses on the isolate (KA/PE1). The patient was treated with polyhexamethylene biguanide, chlorhexidine and oral itraconazole, which resulted in resolution of the patient's ocular inflammation. The majority of the molecular characteristics of the KA/PE1 were determined to be identical, or quite similar, to those of A. castellanii Ma strain, which had been isolated also from amoebic keratitis. The risk of Acanthamoeba keratitis as a potential complication of overnight orthokeratology is briefly discussed.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Astigmatismo
/
Biguanidas
/
Polimorfismo de Fragmento de Restrição
/
DNA Mitocondrial
/
DNA Ribossômico
/
Acanthamoeba
/
RNA Ribossômico 18S
/
Ceratite por Acanthamoeba
/
Clorexidina
/
DNA de Protozoário
Limite:
Animais
/
Feminino
/
Humanos
Idioma:
Inglês
Revista:
The Korean Journal of Parasitology
Ano de publicação:
2006
Tipo de documento:
Artigo
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