A Case of the False-negative D Phenotype in a Neonate with a Strongly Positive Direct Antiglobin Test Rest
Laboratory Medicine Online
;
: 45-49, 2016.
Artigo
em Coreano
| WPRIM
| ID: wpr-220315
ABSTRACT
Accurate D antigen blood typing is needed owing to the clinical importance of the Rh blood group. We describe a female infant who was suspected to suffer from Rh incompatible hemolytic disease of the newborn, and who showed a strong positive direct antiglobin test (DAT) result and false red blood cell (RBC) agglutination in D typing. Using chloroquine dissociation of IgG, we confirmed that the antibodies coating her RBCs were of anti-D type. D typing with 0.8% RBC suspensions in saline using saline gel cards showed 2+ RBC agglutinations. After increasing the incubation time of dissociation by chloroquine for up to 4 hr, the dissociated RBCs began to show agglutination in both the tube technique (2+) and the gel card technique (4+) for D typing, although the DAT rest was still positive. Therefore, in order to prevent mistyping as a false-negative D blood group, whenever the D blood typing of a patient with a strong positive DAT rest does not show RBC agglutination, retesting of the D blood typing is recommended by using saline-suspended RBCs or dissociated RBCs.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fenótipo
/
Suspensões
/
Imunoglobulina G
/
Tipagem e Reações Cruzadas Sanguíneas
/
Cloroquina
/
Aglutinação
/
Eritrócitos
/
Anticorpos
Limite:
Feminino
/
Humanos
/
Lactente
/
Recém-Nascido
Idioma:
Coreano
Revista:
Laboratory Medicine Online
Ano de publicação:
2016
Tipo de documento:
Artigo
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