The Expression of CD 18 on Ischemia-Reperfusion Injury of TRAM Flap of Rats
Journal of the Korean Society of Plastic and Reconstructive Surgeons
;
: 737-741, 2006.
Artigo
em Coreano
| WPRIM
| ID: wpr-220375
ABSTRACT
PURPOSE:
This study was to evaluate the expression pattern of CD 18(leukocyte adhesion glycoprotein) in ischemia-reperfusion injury of TRAM flap of rats. Through this study, we can obtain more information about ischemia-reperfusion injury. We want to develop specific medicine to improve the survival rate of TRAM flap in the future.METHODS:
A TRAM flap supplied by a single pedicle superior epigastric artery and vein was elevated on 60 Sprauge-Dawley rats. The rats were divide into 6 groups (each group n=10); Group O sham, no ischemia-reperfusion injury, Group I 2 hour reperfusion after 4 hour ischemia, Group II 4 hour reperfusion after 4 hour ischemia, Group III 8 hour reperfusion after 4 hour ischemia, Group IV 12 hour reperfusion after 4 hour ischemia, and Group V 24 hour reperfusion after 4 hour ischemia. This study consisted of gross examination for flap survival and flow cytometry study of CD18 on neutrophils.RESULTS:
The gross measurement of the flap showed different survival rate in group I(71%), II(68%), III(37%), IV(34%) and V(34%). All experimental groups showed an increase in the expression of CD18 compared to group O. The expression of CD18 was rapidly increased in ascending order in group I, II and III. But, the expression of CD18 was maintained in group IV and V.CONCLUSION:
The results can be implemented in the study to develop drugs which are capable of reducing ischemia-reperfusion injury in microsurgical breast reconstruction.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Veias
/
Reperfusão
/
Traumatismo por Reperfusão
/
Taxa de Sobrevida
/
Mamoplastia
/
Artérias Epigástricas
/
Citometria de Fluxo
/
Isquemia
/
Neutrófilos
Limite:
Animais
Idioma:
Coreano
Revista:
Journal of the Korean Society of Plastic and Reconstructive Surgeons
Ano de publicação:
2006
Tipo de documento:
Artigo
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