Diagnosis of Fragile X Syndrome and DMD by using DIG System / 대한산부인과학회잡지
Korean Journal of Obstetrics and Gynecology
;
: 2849-2854, 1998.
Artigo
em Coreano
| WPRIM
| ID: wpr-221239
ABSTRACT
The aim of this study was to develop a rapid and safe non-radioactive DIG DNA labeling and detection for Southern blot analysis for fragile X syndrome and Duchenne muscular dystrophy (DMD). Southern blot analysis is accurate test showing expression of the (CGG)n repeat and abnormal methylation pattern of CpG island in hagile X syndrome, and good confirmative secondary test in case of deletion in DMD. But in terms of test rapidity, these conventional radioactive Southern analysis may not be feasible for rapid screening of prenatal samples and at-risk populations to determine their status and to provide genetic counseling to their families. As an alternative radioactive Southern blotting, DIG DNA labeling and detection system does not require handling of radioactive material nor require learning any new technology. The complete procedure of labeling the DNA and hybridization to detection of the first visible signal can be compbsbed witbin 7 days. In addition, hybridization solutions containing labeled DNA can be reused several times after renewed denaturation.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
DNA
/
Programas de Rastreamento
/
Southern Blotting
/
Ilhas de CpG
/
Distrofia Muscular de Duchenne
/
Diagnóstico
/
Síndrome do Cromossomo X Frágil
/
Aconselhamento Genético
/
Aprendizagem
/
Metilação
Tipo de estudo:
Estudo diagnóstico
/
Estudo de rastreamento
Limite:
Humanos
Idioma:
Coreano
Revista:
Korean Journal of Obstetrics and Gynecology
Ano de publicação:
1998
Tipo de documento:
Artigo
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