The Comparison of Parathyroid Hormone Degradation Effect by Various Protease Inhibitors in Blood Specimen / 대한진단검사의학회지
The Korean Journal of Laboratory Medicine
;
: 104-109, 2009.
Artigo
em Inglês
| WPRIM
| ID: wpr-221452
ABSTRACT
BACKGROUND:
The objective of this study was to evaluate the role of proteases on the degradation of parathyroid hormone (PTH) in blood samples.METHODS:
Protease inhibitors with specificity against serine proteases (aprotinin), cysteine proteases (E-64), serine and cysteine proteases (leupeptin), metalloproteases (EDTA), or a protease inhibitor cocktail with a broad spectrum of inhibitory activity were added to blood samples. After storage at room temperature (0-48 hr), PTH levels were measured.RESULTS:
PTH levels in samples with the protease inhibitor cocktail did not change significantly after 48 hr of storage at room temperature, but the average PTH levels decreased by 40.7% and 20.1%, in samples stored at room temperature and stored at 4degrees C without protease inhibitors, respectively. PTH levels in samples with leupeptin were stable for up to 24 hr. After 48 hr, the mean PTH levels decreased by 17.1%, 16.0%, 26.2%, and 32.1%, with 500 KIU/mL aprotinin, 100 micro mol/L leupeptin, 10 micro mol/L E-64, and 10 micro mol/L EDTA, respectively, in the samples stored at room temperature.CONCLUSIONS:
The decrease in PTH levels in blood samples seemed to be due to the degradation of PTH by proteases. Various proteases, including especially serine proteases, would act together to degrade PTH in blood specimen. The PTH degradation may be inhibited in blood specimen with protease inhibitor cocktail.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Hormônio Paratireóideo
/
Inibidores de Proteases
/
Fatores de Tempo
/
Coleta de Amostras Sanguíneas
/
Aprotinina
/
Ácido Edético
/
Leucina
/
Leupeptinas
Limite:
Feminino
/
Humanos
/
Masculino
Idioma:
Inglês
Revista:
The Korean Journal of Laboratory Medicine
Ano de publicação:
2009
Tipo de documento:
Artigo
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