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Simultaneous HPLC Analysis of Three Flavonoids in the Extracts of Artocarpus heterophyllus Heartwoods
Natural Product Sciences ; : 77-81, 2016.
Artigo em Inglês | WPRIM | ID: wpr-221639
ABSTRACT
A reversed-phase high-performance liquid chromatographic method is described for the simultaneous determination of three antibacterial flavonoids, artocarpanone, artocarpin, and cycloartocarpin in ethyl acetate extracts from Artocarpus heterophyllus heartwoods. Separation was achieved using a TSK-gel ODS-80Tm column (5 µm, 4.6 × 150 mm) at 25℃ with a gradient elution system of methanol and water as follows 0-8 min, 6040; 8-27 min, 8020; 27-35 min, 6040, v/v, at a flow rate of 1 mL/min, and a quantitative UV detection at 285 nm. The method was validated by measuring the key parameters, including specificity, linearity, sensitivity, accuracy, repeatability and reproducibility. A high degree of specificity and sensitivity was achieved. The calibration curves for all three flavonoids showed good linearity with a coefficient of determinations (R²) of ≥ 0.9995. The recoveries of the method were from 98-104%, with good reproducibility and repeatability (RSD values of less than 2%) were also achieved. Ethyl acetate was the best solvent for extraction of these three flavonoids using the heat reflux conditions for 1 h. This optimized sample preparation and HPLC method can be practically used for a routine standardization process of the extracts from the A. heterophyllus heartwoods.
Assuntos

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Flavonoides / Calibragem / Água / Cromatografia Líquida de Alta Pressão / Sensibilidade e Especificidade / Artocarpus / Metanol / Temperatura Alta / Métodos Tipo de estudo: Estudo diagnóstico Idioma: Inglês Revista: Natural Product Sciences Ano de publicação: 2016 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Flavonoides / Calibragem / Água / Cromatografia Líquida de Alta Pressão / Sensibilidade e Especificidade / Artocarpus / Metanol / Temperatura Alta / Métodos Tipo de estudo: Estudo diagnóstico Idioma: Inglês Revista: Natural Product Sciences Ano de publicação: 2016 Tipo de documento: Artigo