MicroRNA Analysis during Cultured Odontoblast Differentiation
International Journal of Oral Biology
; : 146-152, 2012.
Article
em Ko
| WPRIM
| ID: wpr-222606
Biblioteca responsável:
WPRO
ABSTRACT
MicroRNAs (miRNAs, miRs) are about 21-25 nucleotides in length and regulate mRNA translation by base pairing to partially complementary sites, predominantly in the 3'-untranslated region (3'-UTR) of the target mRNA. In this study, the expression profile of miRNAs was compared and analyzed for the establishment of miRNA-related odontoblast differentiation using MDPC-23 cells derived from mouse dental papilla cells. To determine the expression profile of miRNAs during the differentiation of MDPC-23 cells, we employed miRNA microarray analysis, quantitative real-time PCR (qRT-PCR) and Alizaline red-S staining. In the miRNA microarray analysis, 11 miRNAs were found to be up- or down-regulated more than 3-fold between day 0 (control) and day 5 of MDPC-23 cell differentiation among the 1,769 miRNAs examined. In qRT-PCR analysis, the expression levels of two of these molecules, miR-194 and miR-126, were increased and decreased in the control MDPC-23 cells compared with the MDPC-23 cells at day 5 of differentiation, respectively. Importantly, the overexpression of miR-194 significantly accelerated mineralization compared with the control cultures during the differentiation of MDPC-23 cells. These results suggest that the miR-194 augments MDPC-23 cell differentiation, and potently accelerates the mineralization process. Moreover, these in vitro results show that different miRNAs are deregulated during the differentiation of MDPC-23 cells, suggesting the involvement of these genes in the differentiation and mineralization of odontoblasts.
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Índice:
WPRIM
Assunto principal:
Biossíntese de Proteínas
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RNA Mensageiro
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Diferenciação Celular
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Pareamento de Bases
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MicroRNAs
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Papila Dentária
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Análise em Microsséries
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Reação em Cadeia da Polimerase em Tempo Real
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Nucleotídeos
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Odontoblastos
Limite:
Animals
Idioma:
Ko
Revista:
International Journal of Oral Biology
Ano de publicação:
2012
Tipo de documento:
Article