Dexmedetomidine attenuates H₂O₂-induced cell death in human osteoblasts
Journal of Dental Anesthesia and Pain Medicine
;
: 295-302, 2016.
Artigo
em Inglês
| WPRIM
| ID: wpr-222965
ABSTRACT
BACKGROUND:
Reactive oxygen species play critical roles in homeostasis and cell signaling. Dexmedetomidine, a specific agonist of the α₂-adrenoceptor, has been commonly used for sedation, and it has been reported to have a protective effect against oxidative stress. In this study, we investigated whether dexmedetomidine has a protective effect against H₂O₂-induced oxidative stress and the mechanism of H₂O₂-induced cell death in normal human fetal osteoblast (hFOB) cells.METHODS:
Cells were divided into three groups control group—cells were incubated in normoxia without dexmedetomidine, hydrogen peroxide (H2O2) group—cells were exposed to H₂O₂ (200 µM) for 2 h, and Dex/H₂O₂ group—cells were pretreated with dexmedetomidine (5 µM) for 2 h then exposed to H₂O₂ (200 µM) for 2 h. Cell viability and apoptosis were evaluated. Osteoblast maturation was determined by assaying bone nodular mineralization. Expression levels of bone-related proteins were determined by western blot.RESULTS:
Cell viability was significantly decreased in the H₂O₂ group compared with the control group, and this effect was improved by dexmedetomidine. The Hoechst 33342 and Annexin-V FITC/PI staining revealed that dexmedetomidine effectively decreased H₂O₂-induced hFOB cell apoptosis. Dexmedetomidine enhanced the mineralization of hFOB cells when compared to the H₂O₂ group. In western blot analysis, bone-related protein was increased in the Dex/H₂O₂ group.CONCLUSIONS:
We demonstrated the potential therapeutic value of dexmedetomidine in H₂O₂-induced oxidative stress by inhibiting apoptosis and enhancing osteoblast activity. Additionally, the current investigation could be evidence to support the antioxidant potential of dexmedetomidine in vitro.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Osteoblastos
/
Técnicas In Vitro
/
Sobrevivência Celular
/
Western Blotting
/
Morte Celular
/
Espécies Reativas de Oxigênio
/
Apoptose
/
Estresse Oxidativo
/
Dexmedetomidina
/
Mineradores
Limite:
Humanos
Idioma:
Inglês
Revista:
Journal of Dental Anesthesia and Pain Medicine
Ano de publicação:
2016
Tipo de documento:
Artigo
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