Ginkgolide B Modulates BDNF Expression in Acute Ischemic Stroke
Journal of Korean Neurosurgical Society
;
: 391-396, 2017.
Artigo
em Inglês
| WPRIM
| ID: wpr-224196
ABSTRACT
OBJECTIVE:
To investigate the neuroprotective effects of Ginkgolide B (GB) against ischemic stroke-induced injury in vivo and in vitro, and further explore the possible mechanisms concerned.METHODS:
Transient middle cerebral artery occlusion (tMCAO) mice and oxygen-glucose deprivation/reoxygenation (OGD/R)-treated N2a cells were used to explore the neuroprotective effects of GB. The expression of brain-derived neurotrophic factor (BDNF) was detected via Western blot and qRT-PCR.RESULTS:
GB treatment (4 mg/kg, i. p., bid) significantly reduced neurological deficits, water content, and cerebral infarct volume in tMCAO mice. GB also significantly increased Bcl-2/Bax ratio, reduced the expression of caspase-3, and protected against OGD/R-induced neuronal apoptosis. Meanwhile, GB caused the up-regulation of BDNF protein in vivo and in vitro.CONCLUSION:
Our data suggest that GB might protect the brain against ischemic insult partly via modulating BDNF expression.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Técnicas In Vitro
/
Encéfalo
/
Água
/
Regulação para Cima
/
Western Blotting
/
Apoptose
/
Fármacos Neuroprotetores
/
Fator Neurotrófico Derivado do Encéfalo
/
Acidente Vascular Cerebral
/
Infarto da Artéria Cerebral Média
Limite:
Animais
Idioma:
Inglês
Revista:
Journal of Korean Neurosurgical Society
Ano de publicação:
2017
Tipo de documento:
Artigo
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