Identification of eplication-ompetent Dimeric Human Hepatitis B Viral Using PCR Screening Technique / 대한간학회지
The Korean Journal of Hepatology
;
: 184-189, 1999.
Artigo
em Coreano
| WPRIM
| ID: wpr-224749
ABSTRACT
BACKGROUND/AIMS:
Transfection of the hepatitis B virus (HBV) genome requires the cloning of tandem HBV sequences inserted into a plasmid vector, which is usually screened for by the restriction enzyme digestion of plasmid minipreparation from at least a dozen of bacterial colonies. The aim of this study was to develop a simple alternative screening method for bacterial colonies harbouring tandem HBV sequences by a PCR.METHODS:
A set of polymerase chain reaction (PCR) primer was designed to detect the bacterial colonies harbouring "head to tail" dimeric HBV DNA. PCR which amplifies the head to tail junction site of two tandem HBV molecules was performed.RESULTS:
PCR products with appropriate size (1.2kb) were obtained. The accurate detection by PCR screening technique was confirmed by enzyme digestion.CONCLUSIONS:
These results suggest that PCR screening technique is a simple and rapid method for the identification of bacterial colonies containing tandem HBV sequences.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Plasmídeos
/
DNA
/
Transfecção
/
Vírus da Hepatite B
/
Programas de Rastreamento
/
Reação em Cadeia da Polimerase
/
Genoma
/
Células Clonais
/
Clonagem de Organismos
/
Digestão
Tipo de estudo:
Estudo diagnóstico
/
Estudo de rastreamento
Limite:
Humanos
Idioma:
Coreano
Revista:
The Korean Journal of Hepatology
Ano de publicação:
1999
Tipo de documento:
Artigo
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