Prevention of Virus - induced Diabetes by Single Immunization with Recombinant BCG in SJL/J Male Mice / 대한면역학회지
Korean Journal of Immunology
;
: 259-269, 1999.
Artigo
em Coreano
| WPRIM
| ID: wpr-224754
ABSTRACT
D variant of encephalomyocarditis (EMC-D) virus causes diabetes in susceptible mice by direct infection and cytolysis of pancreatic beta cells. cDNA covering the major outer capsid protein (VP1) of EMC-D virus was cloned into Mycobacterium bovis bacillus Calmette-Guerin (BCG). None of the SJL/J male mice, immunized with live recombinant BCG-VP1, became diabetic when challenged with highly diabetogenic EMC-D virus. But the control mice inoculated with normal BCG or rBCG transformed with vector alone developed diabetes in the same challenge. VP1-specific antibodies including neutralizing antibodies were markedly increased as time went on and reached to the maximum titer at week 10 after a single immunization. The plateau of the titer lasted longer than following 4 weeks. Guinea pigs immunized with the live rBCG-VP1 showed strong delayed type hypersensitivity (DTH) to the VP1of EMC-D virus. It means that the live rBCG-VP1 elicit efficient humoral and cell-mediated imrnune responses against EMC-D virus, resulting in prevention of virus-induced diabetes in susceptible mice.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Bacillus
/
Imunização
/
Células Clonais
/
DNA Complementar
/
Proteínas do Capsídeo
/
Células Secretoras de Insulina
/
Anticorpos Neutralizantes
/
Cobaias
/
Hipersensibilidade
/
Anticorpos
Limite:
Animais
/
Humanos
/
Masculino
Idioma:
Coreano
Revista:
Korean Journal of Immunology
Ano de publicação:
1999
Tipo de documento:
Artigo
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