A Study of the Pathway of Nitric Oxide Production by Nitroglycerin in Trabecular Meshwork Cells
Journal of the Korean Ophthalmological Society
;
: 1429-1434, 2013.
Artigo
em Coreano
| WPRIM
| ID: wpr-225268
ABSTRACT
PURPOSE:
To investigate the effects of nitroglycerin on the production of nitric oxide and its related pathway in cultured human trabecular meshwork cells (HTMC).METHODS:
Primarily cultured HTMC were exposed to 10 nM nitroglycerin using 1% serum-containing media for 30 minutes. The production of nitric oxide was assessed with the Griess assay and expressions of eNOS mRNA was assessed with RT-PCR. Additionally, the cells were exposed to wortmanin and Akt1/2 kinase inhibitor to investigate the mechanism related to the production of nitric oxide.RESULTS:
Nitroglycerin increased the production of nitric oxide (p < 0.05) accompanied with increased expression of eNOS mRNA. The increased production of nitric oxide and eNOS mRNA was inhibited by wortmanin and Akt1/2 kinase inhibitor.CONCLUSIONS:
Low-dose nitroglycerin increased the production of nitric oxide accompanied by increased eNOS activity. Nitroglycerin drives eNOS activation via the phosphatidylinositol 3-kinase/protein kinase B pathway.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fosfatidilinositóis
/
Fosfotransferases
/
Malha Trabecular
/
RNA Mensageiro
/
Nitroglicerina
/
Óxido Nítrico
Limite:
Humanos
Idioma:
Coreano
Revista:
Journal of the Korean Ophthalmological Society
Ano de publicação:
2013
Tipo de documento:
Artigo
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