A Comparative Study to Determine the Most Suitable Fixative for Immunocytochemical Staining of Cytospin Slides / 임상검사와정도관리
Journal of Laboratory Medicine and Quality Assurance
;
: 13-22, 2013.
Artigo
em Coreano
| WPRIM
| ID: wpr-225312
ABSTRACT
BACKGROUND:
Fixation of cells is a critical procedure that can determine the success of immunocytochemical staining (ICC) of cytospin slides. In this study, we evaluated the efficacy of a number of fixatives to determine the ideal fixative for ICC of cytospin slides.METHODS:
Sixteen patients with metastatic neoplasm in the body cavity were enrolled. Cytospin slides were prepared from each patient using 5 different fixatives (cold acetone, 95% ethanol, 11 methanolethanol, 3.7% formalin, and 31 methanolacetone), and the suitability of each for use with Wright's stain was compared. For 4 of the samples, appropriate ICCs were performed using all 5 fixatives and the results were compared, while for 11 samples, only the first 3 fixatives were used for ICC.RESULTS:
Using Wright stain, cold acetone, 95% ethanol, and 11 methanolethanol fixatives all showed similar efficacy when compared to the conventional methanol fixation method. However, the stain quality using 3.7% formalin or 31 methanolacetone fixatives was poor due to deterioration of cell adhesion and distortion of cell morphology. Using ICC, cold acetone fixative showed stronger and more tumor-specific stainability than the 95% ethanol (decreased stainability in 6 stained slides, false positive staining of histiocytes/neutrophils in 4 stained slides, no staining of CD3 and terminal deoxynucleotidyl transferase [TdT]) and 11 methanolethanol fixatives (decreased stainability in 3 stained slides, false positive staining of histiocytes/neutrophils in 2 stained slides, no staining of CD3 and TdT).CONCLUSIONS:
Cold acetone fixative was the most efficacious among the 5 fixatives tested in this study; therefore, it is the most appropriate fixative in the preparation of cytospin slides for ICC.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Acetona
/
Imuno-Histoquímica
/
Adesão Celular
/
Temperatura Baixa
/
Etanol
/
DNA Nucleotidilexotransferase
/
Metanol
/
Fixadores
/
Formaldeído
Limite:
Humanos
Idioma:
Coreano
Revista:
Journal of Laboratory Medicine and Quality Assurance
Ano de publicação:
2013
Tipo de documento:
Artigo
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