Isolation of Middle East Respiratory Syndrome Coronavirus from a Patient of the 2015 Korean Outbreak
Journal of Korean Medical Science
;
: 315-320, 2016.
Artigo
em Inglês
| WPRIM
| ID: wpr-225574
ABSTRACT
During the 2015 outbreak of Middle East respiratory syndrome coronavirus (MERS-CoV) in Korea, 186 persons were infected, resulting in 38 fatalities. We isolated MERS-CoV from the oropharyngeal sample obtained from a patient of the outbreak. Cytopathic effects showing detachment and rounding of cells were observed in Vero cell cultures 3 days after inoculation of the sample. Spherical virus particles were observed by transmission electron microscopy. Full-length genome sequence of the virus isolate was obtained and phylogenetic analyses showed that it clustered with clade B of MERS-CoV.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Filogenia
/
Células Vero
/
RNA Viral
/
Microscopia Eletrônica
/
Chlorocebus aethiops
/
Reação em Cadeia da Polimerase
/
Surtos de Doenças
/
Análise de Sequência de RNA
/
Infecções por Coronavirus
/
República da Coreia
Limite:
Animais
/
Humanos
País/Região como assunto:
Ásia
Idioma:
Inglês
Revista:
Journal of Korean Medical Science
Ano de publicação:
2016
Tipo de documento:
Artigo
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