Adrenalectomy Abolishes Fasting-induced Down-regulation of NADPH-diaphorase in the Rat Paraventricular Nucleus
Yonsei Medical Journal
;
: 123-128, 2004.
Artigo
em Inglês
| WPRIM
| ID: wpr-225870
ABSTRACT
This study was conducted to define the molecular mechanism of fasting-induced down-regulation of neuronal nitric oxide synthase (nNOS) expression in the hypothalamic paraventricular nucleus (PVN). Rats were adrenalectomized (ADX), and then either underwent food deprivation or received varying doses of dexamethasone for 48 h. The brain tissues were processed for NADPH-diaphorase (NADPH-d) staining, a histochemical marker of nNOS enzyme activity. Both the ADX and the sham operated rats showed a significant weight loss after 48 h of food deprivation. Food deprivation decreased the number of NADPH-d containing cells in the PVN of sham rats, however, not in the ADX rats. Dexamethasone dose- dependently decreased NADPH-d cells in the PVN of ADX rats. The effect of ADX or dexamethasone was limited to the parvocellular subdivision of PVN. These results suggest that the adrenal glucocorticoids may down-regulate nNOS expression in the PVN during food deprivation.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Núcleo Hipotalâmico Paraventricular
/
Dexametasona
/
Biomarcadores
/
Regulação para Baixo
/
Jejum
/
Ratos Sprague-Dawley
/
Adrenalectomia
/
Óxido Nítrico Sintase
/
Privação de Alimentos
/
Glucocorticoides
Limite:
Animais
Idioma:
Inglês
Revista:
Yonsei Medical Journal
Ano de publicação:
2004
Tipo de documento:
Artigo
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