The Role of Chronic Endothelin Antagonism for Preserving Erectile Function in Experimental Diabetic Rats / 대한남성과학회지
Korean Journal of Andrology
;
: 75-81, 2002.
Artigo
em Coreano
| WPRIM
| ID: wpr-226048
ABSTRACT
PURPOSE:
This study was designed to investigate the role of chronic endothelin receptor antagonism to preserve erectile function in diabetic rats. MATERIALS ANDMETHODS:
Forty adult male Sprague-Dawley rats (250-350 gm) were divided into 4 groups Group 1, non-diabetic control rats (n=5); Group 2, non-diabetic rats fed 10 mg/kg of RO-485695, a combined endothelin receptor antagonist (n=5); Group 3, diabetic control (n=15); Group 4, diabetic rats fed 10 mg/kg of RO-485695 (n=15). Streptozotocin, 65 mg/kg, was used for development of diabetes mellitus. Body weight and blood glucose levels were measured every 2 weeks and drug feeding was done by oral gavage. Twelve weeks after induction of diabetes and RO-485695 treatment, erectile function was determined by measurement of intracavernosal pressure (ICP) and maximal arterial pressure (MAP) after electrical stimulation of the cavernosal nerve. RT-PCR analysis for detection of mRNA of endothelin-1, endothelin receptor A (ET-A), and endothelin receptor B (ET-B), and Western blot analysis and immunohistochemical evaluation of the eNOS protein were performed.RESULTS:
Body weight and blood glucose levels were not influenced by 10 mg/kg of RO-485695. Erectile function after 5 volts stimulation was significantly decreased in the diabetic rat (group 3 & 4) compared with the non-diabetic rat (group 1 & 2) (64.3+/-9.6 vs 41.9+/-14.8 mmHg), but increased in group 4 compared with group 3 (44.3+/-16.9 vs 36.6+/-10.1 mmHg). The mRNA expression of endothelin-1 was up-regulated significantly in group 3 & 4 when compared with group 1 & 2. However this endothelin expression was down-regulated in group 4 compared with group 3. The mRNA expression of ET-A among 4 groups was not significantly different. ET-B band in rat cavernosal tissue could not be observed in any group under these conditions. eNOS protein expression was increased in group 4 compared with group 3.CONCLUSIONS:
Chronic endothelin receptor antagonism in the experimental diabetic rat model has been demonstrated to preserve cavernosal erectile function, suggesting a possible therapeutic role for endothelin receptor antagonists in diabetic erectile dysfunction.
Texto completo:
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Índice:
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Assunto principal:
Glicemia
/
Peso Corporal
/
RNA Mensageiro
/
Western Blotting
/
Endotelinas
/
Receptores de Endotelina
/
Ratos Sprague-Dawley
/
Estreptozocina
/
Endotelina-1
/
Modelos Animais
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
/
Humanos
/
Masculino
Idioma:
Coreano
Revista:
Korean Journal of Andrology
Ano de publicação:
2002
Tipo de documento:
Artigo
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