Focal cerebral ischemic injury decreases calbindin expression in brain tissue and HT22 cells / 한국실험동물학회지
Laboratory Animal Research
;
: 156-161, 2013.
Artigo
em Inglês
| WPRIM
| ID: wpr-226194
ABSTRACT
Calbindin is a calcium binding protein that controls intracellular calcium levels and has a neuroprotective function against apoptotic stimuli. We investigated the expression of calbindin in ischemic brain injury. Focal cerebral ischemia was induced in male rats by middle cerebral artery occlusion (MCAO) and cerebral cortices were collected 24 h after MCAO. Cerebral ischemia significantly increased infarct volume. RT-PCR and Western blot analyses showed that MCAO injury induced a decrease of calbindin expression. Moreover, immunohistochemical staining showed that the number of calbindin-positive cells decreased in ischemic regions of MCAO-operated animals. In cultured hippocampal-derived cell lines, glutamate exposure increased intracellular Ca2+ concentrations and decreased calbindin expression. Taken together, both in vivo and in vitro results demonstrated decreases of calbindin after neuronal cell injury. These results suggest that decreases of calbindin in ischemic brain injury contribute to neuronal cell death.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Encéfalo
/
Lesões Encefálicas
/
Proteína G de Ligação ao Cálcio S100
/
Proteínas de Transporte
/
Linhagem Celular
/
Córtex Cerebral
/
Isquemia Encefálica
/
Western Blotting
/
Cálcio
/
Morte Celular
Limite:
Animais
/
Humanos
/
Masculino
Idioma:
Inglês
Revista:
Laboratory Animal Research
Ano de publicação:
2013
Tipo de documento:
Artigo
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