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Identification of Gemella species by 16S ribosomal RNA gene sequencing from two patients with infective endocarditis / 대한내과학회지
Korean Journal of Medicine ; : 591-596, 2006.
Artigo em Coreano | WPRIM | ID: wpr-227049
ABSTRACT
Gemella species are opportunistic pathogens, which cause endocarditis, meningitis, musculoskeletal infections, or pulmonary infections. It is difficult to identify all strains of these species by a conventional culture system. Because 16S rRNA gene has the interspecific polymorphisms to differentiate the bacterial species, 16S rRNA gene sequence analysis could be used to identify these pathogens. To determine the molecular types of two unidentified Gram-positive cocci from two patients with infective endocarditis, PCR amplification and DNA sequencing of the 16S rRNA gene were performed. These sequences of the PCR product were compared with known 16S rRNA gene sequences using GenBank BLAST search. The 16S rRNA gene sequences of two isolates showed > 99% nucleotide similarities with those of Gemella morbillorum (GenBank accession number L14327). Phylogenetic analysis also indicated the close relatedness between these isolates and G. morbillorum.
Assuntos

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: RNA Ribossômico 16S / Reação em Cadeia da Polimerase / Cocos Gram-Positivos / Análise de Sequência de DNA / Análise de Sequência / Genes de RNAr / Bases de Dados de Ácidos Nucleicos / Endocardite / Gemella / Meningite Tipo de estudo: Estudo diagnóstico Limite: Humanos Idioma: Coreano Revista: Korean Journal of Medicine Ano de publicação: 2006 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: RNA Ribossômico 16S / Reação em Cadeia da Polimerase / Cocos Gram-Positivos / Análise de Sequência de DNA / Análise de Sequência / Genes de RNAr / Bases de Dados de Ácidos Nucleicos / Endocardite / Gemella / Meningite Tipo de estudo: Estudo diagnóstico Limite: Humanos Idioma: Coreano Revista: Korean Journal of Medicine Ano de publicação: 2006 Tipo de documento: Artigo