Your browser doesn't support javascript.
loading
Role of annexin-I in cervical cancer cell proliferation / 대한산부인과학회잡지
Korean Journal of Obstetrics and Gynecology ; : 2199-2204, 1999.
Artigo em Coreano | WPRIM | ID: wpr-227086
ABSTRACT

OBJECTIVES:

To investigate the role of annexin-I in human cervical cancer, we evaluated the expression of annexin-I and the relation with the proliferation of cancer cells.

Methods:

By immunohistochemical analysis and the western blotting of annexin-I , we investigated the extent and distribution of the expression of annexin-I in cervical cancer tissues. After treating the human cancer cell lines ( SiHa and HeLa cell lines ) with tamoxifen, estradiol, and retinoic acid for 5 days to make the cells proliferate and antiproliferate, we measured the proliferation simultaneously with 3-(4,5- dimethyl thiazol- 2-yl)-2,5-diphenyl-tetr -azolium bromide (MTT) colorimetric assay and the expression level of annexin-I with flowcytometry.

RESULTS:

In the immunohistochemical stains, a granular staining pattern involving the entire cytoplasm was more heavily observed in malignant lesions than in normals. In the western blotting, the antibodies against 35-kDa annexin-I appeared to react more strongly with the lysates of cancer tissues than normal and benign tissues. In SiHa and HeLa cell lines with tamoxifen and beta- estradiol treatment, increased expressions of annexin-I were noted with correlated increased proliferation of cells, and with the treatments of all trans retinoic acid, decreased expressions of annexin-I were noted with correlated decreased proliferation of cells.

CONCLUSIONS:

The results suggest that the expression of annexin-I might correlate with cervical cancer than normal and the proliferation of cancer cells.
Assuntos

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Tamoxifeno / Tretinoína / Células HeLa / Linhagem Celular / Neoplasias do Colo do Útero / Western Blotting / Citoplasma / Proliferação de Células / Corantes / Estradiol Limite: Humanos Idioma: Coreano Revista: Korean Journal of Obstetrics and Gynecology Ano de publicação: 1999 Tipo de documento: Artigo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Tamoxifeno / Tretinoína / Células HeLa / Linhagem Celular / Neoplasias do Colo do Útero / Western Blotting / Citoplasma / Proliferação de Células / Corantes / Estradiol Limite: Humanos Idioma: Coreano Revista: Korean Journal of Obstetrics and Gynecology Ano de publicação: 1999 Tipo de documento: Artigo