Determination of Oxyclozanide in Beef and Milk using High-Performance Liquid Chromatography System with UV Detector / 한국실험동물학회지
Laboratory Animal Research
; : 37-40, 2011.
Article
em En
| WPRIM
| ID: wpr-227296
Biblioteca responsável:
WPRO
ABSTRACT
This study was developed and validated for the determination of oxyclozanide residue concentrations in beef and commercial milk, using high-performance liquid chromatography system. Oxyclozanide was successfully separated on a reverse phase column (Xbridge-C18, 4.6x250 mm, 5 microm) with a mobile phase composed of acetonitrile and 0.1% phosphoric acid (60:40, v/v%). This analytical procedure involved a deproteinization process using acetonitrile for beef and 2% formic acid in acetonitrile for commercial milk, dehydration by adding sodium sulfate to the liquid analytical sample, and a defatting process using n-hexane; after these steps, the extract was exposed to a stream of nitrogen dryness. The final extracted sample was dissolved in the mobile phase and filtered using a 0.45 microm syringe filter. This method had good selectivity and recovery (70.70+/-7.90-110.79+/-14.95%) from the matrices. The LOQs ranged from 9.7 to 9.8 microg/kg for beef and commercial milk. The recoveries met the standards set by the CODEX guideline.
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Texto completo:
1
Índice:
WPRIM
Assunto principal:
Oxiclozanida
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Acetonitrilas
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Ácidos Fosfóricos
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Sódio
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Sulfatos
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Seringas
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Cromatografia Líquida de Alta Pressão
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Cromatografia Líquida
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Desidratação
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Rios
Idioma:
En
Revista:
Laboratory Animal Research
Ano de publicação:
2011
Tipo de documento:
Article