Change in the Gene Expression of HOS Cell Line by 17beta-Estradiol -Study using cDNA Array- / 대한정형외과연구학회지

ABSTRACT

Osteoporosis is caused by the decrease in bone mass associated with disturbance in bone remodeling. Bone remodeling depends on the spatial and temporal coupling of bone formation by osteoblasts and bone resorption by osteoclasts. However, the molecular basis of these inductive interactions is unknown. To establish a new way of study on the function of osteoblast, the profile of genes expressed in proliferating osteoblast cell line was investigated using cDNA array of 588 genes. Among 232 genes expressed in HOS cell line, the most abundantly expressed gene was 40S ribosomal protein S19. The glutathione S-transferase pi gene and thymosin beta-10 gene were expressed highly also. The expressed genes were 23 oncogenes and tumor suppressors, genes for 20 cell cycle control proteins, 1 ion channels, 30 modulators/effectors/intracellular transducers, 6 stress response proteins, 24 apoptosis-related protein, 16 DNA synthesis/DNA repair/recombination proteins, 36 DNA binding/transcription factors, 35 cell receptorsl, and 41 extracellular cell signalling and communication proteins. The expressions of ten genes were changed by treatment of 17beta-estradiol. Eight genes were overexpressed and two genes were down-regulated by 17beta-estradiol. These results show the expression profile of genes in proliferating osteoblast cell line and show ten genes regulated by 17beta-estradiol.