The Cell Cycle Regulatory Effects of High Dose 5-fluorouracil on Breast Cancer Cell Line
Immune Network
;
: 60-64, 2002.
Artigo
em Coreano
| WPRIM
| ID: wpr-228524
ABSTRACT
BACKGROUND:
Chemotherapy with 5-fluorouracil (5-FU) has been one of the mainstay in breast cancer treatment. The effects of high dose 5-FU on cell cycle regulation were studied in breast caner cells.METHODS:
A breast cancer cell line MCF-7 was used. Protein expressions of G1/S cyclins, p21(Waf1/Cip1), cdk2, E2F1 and retinoblastoma were tested by western blot analysis. Immunoprecipitation and immune complex kinase assay were done for the assessment of E2F1/RB interacton and the activity of cdk2 respectively.RESULTS:
p21(Waf1/Cip1) expression was barely detectable in control cells. With addition of 5-FU level of p21(Waf1/Cip1) were induced and cyclin D3 level was decreased as cell growth decreases. In accordance with increased expression of p21(Waf1/Cip1), cyclin E-associated cdk2 kinase activity was reduced. Retinoblastoma protein (RB) became dephosphorylated and E2F-1 binding activity with RB was increased.CONCLUSION:
In this situation of high concentration of 5-FU breast cancer cells tend to be G1/S cell cycle arrested. Overexpression of p21(Waf1/Cip1) and dephosphorylation of RB may mediate the effectss of 5-FU by inhibiting E2F-1 activity, which contributes to G1/S cell cycle arrest. These results could be an indicating landmark for further study of high dose chemotherapy with 5-FU.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fosfotransferases
/
Retinoblastoma
/
Mama
/
Neoplasias da Mama
/
Ciclo Celular
/
Linhagem Celular
/
Western Blotting
/
Proteína do Retinoblastoma
/
Ciclinas
/
Imunoprecipitação
Idioma:
Coreano
Revista:
Immune Network
Ano de publicação:
2002
Tipo de documento:
Artigo
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