Trichomonas vaginalis Metalloproteinase Induces mTOR Cleavage of SiHa Cells
The Korean Journal of Parasitology
;
: 595-603, 2014.
Artigo
em Inglês
| WPRIM
| ID: wpr-229078
ABSTRACT
Trichomonas vaginalis secretes a number of proteases which are suspected to be the cause of pathogenesis; however, little is understood how they manipulate host cells. The mammalian target of rapamycin (mTOR) regulates cell growth, cell proliferation, cell motility, cell survival, protein synthesis, and transcription. We detected various types of metalloproteinases including GP63 protein from T. vaginalis trophozoites, and T. vaginalis GP63 metalloproteinase was confirmed by sequencing and western blot. When SiHa cells were stimulated with live T. vaginalis, T. vaginalis excretory-secretory products (ESP) or T. vaginalis lysate, live T. vaginalis and T. vaginalis ESP induced the mTOR cleavage in both time- and parasite load-dependent manner, but T. vaginalis lysate did not. Pretreatment of T. vaginalis with a metalloproteinase inhibitor, 1,10-phenanthroline, completely disappeared the mTOR cleavage in SiHa cells. Collectively, T. vaginalis metallopeptidase induces host cell mTOR cleavage, which may be related to survival of the parasite.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Trichomonas vaginalis
/
Western Blotting
/
Análise de Sequência de DNA
/
Linhagem Celular Tumoral
/
Metaloproteases
/
Células Epiteliais
/
Serina-Treonina Quinases TOR
/
Proteólise
Limite:
Humanos
Idioma:
Inglês
Revista:
The Korean Journal of Parasitology
Ano de publicação:
2014
Tipo de documento:
Artigo
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