Molecular cloning of a novel cDNA from Mus muscular BALB/c mice encoding glycosyl hydrolase family 1: a homolog of human lactase-phlorizin hydrolase / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 340-345, 2006.
Artigo
em Inglês
| WPRIM
| ID: wpr-229678
ABSTRACT
<p><b>OBJECTIVE</b>To study the mechanism of lactose intolerance (LI) by cloning the mouse lactase cDNA and recombining a vector. METHODS Total murine RNA was isolated from the small intestine of a 4-week-old BALB/c mouse (d). Gene-specific primers were designed and synthesized according to the cDNA sequences of lactase-phlorizin hydrolase (LPH) in human, rat, and rabbit. A coding sequence (CDS) fragment was obtained using RT-PCR, and inserted into a clone vector pNEB-193, then the cDNA was sequenced and analyzed using bioinformatics.</p><p><b>RESULTS</b>The cDNA from the BALB/c mouse with 912 bp encoding 303 amino acid residues. Analysis of the deduced amino acid sequence using bioinformatics revealed that this cDNA shared extensive sequence homology with human LPH containing a conserved glycosyl hydrolase family 1 motif important for regulating lactase intolerance.</p><p><b>CONCLUSION</b>BALB/c mouse LPH cDNA (GenBank accession No AY751548) provides a necessary foundation for study of the biological function and regulatory mechanism of the lactose intolerance in mice.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Clonagem Molecular
/
Lactase-Florizina Hidrolase
/
DNA Complementar
/
Genética
/
Camundongos Endogâmicos BALB C
Limite:
Animais
/
Humanos
Idioma:
Inglês
Revista:
Biomedical and Environmental Sciences
Ano de publicação:
2006
Tipo de documento:
Artigo
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