Bone marrow stromal cells express neural phenotypes in vitro and migrate in brain after transplantation in vivo / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 329-335, 2006.
Artigo
em Inglês
| WPRIM
| ID: wpr-229680
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the differentiation of bone marrow stromal cells (BMSC) into neuron-like cells and to explore their potential use for neural transplantation.</p><p><b>METHODS</b>BMSC from rats and adult humans were cultured in serum-containing media. Salvia miltiorrhiza was used to induce human BMSC (hBMSC) to differentiate. BMSC were identified with immunocytochemistry. Semi-quantitative RT-PCR was used to examine mRNA expression of neurofilamentl (NF1), nestin and neuron-specific enolase (NSE) in rat BMSC (rBMSC). Rat BMSC labelled by Hoschst33258 were transplanted into striatum of rats to trace migration and distribution.</p><p><b>RESULTS</b>rBMSC expressed NSE, NF1 and nestin mRNA, and NF1 mRNA and expression was increased with induction of Salvia miltiorrhiza. A small number of hBMSC were stained by anti-nestin, anti-GFAP and anti-S100. Salvia miltiorrhiza could induce hBMSC to differentiate into neuron-like cells. Some differentiated neuron-like cells, that expressed NSE, beta-tubulin and NF-200, showed typical neuron morphology, but some neuron-like cells also expressed alpha smooth muscle protein, making their neuron identification complicated. rBMSC could migrate and adapted in the host brains after being transplanted.</p><p><b>CONCLUSION</b>Bone marrow stromal cells could express phenotypes of neurons, and Salvia miltiorrhiza could induce hBMSC to differentiate into neuron-like cells. If BMSC could be converted into neurons instead of mesenchymal derivatives, they would be an abundant and accessible cellular source to treat a variety of neurological diseases.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Transplante
/
Encéfalo
/
Medicamentos de Ervas Chinesas
/
Extratos Vegetais
/
Diferenciação Celular
/
Células Cultivadas
/
Transplante de Medula Óssea
/
Células Estromais
/
Ratos Sprague-Dawley
Limite:
Animais
/
Humanos
/
Masculino
Idioma:
Inglês
Revista:
Biomedical and Environmental Sciences
Ano de publicação:
2006
Tipo de documento:
Artigo
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