Establishment of cell free conversion system with biotin-labelled recombinant PrPsen expressed in E. coli / 生物医学与环境科学（英文）
Biomedical and Environmental Sciences
;
(12): 214-218, 2006.
Artigo
em Inglês
| WPRIM
| ID: wpr-229699
ABSTRACT
<p><b>OBJECTIVE</b>To report a protocol using biotin-labelled PrP protein in cell free conversion assay instead of isotope.</p><p><b>METHODS</b>A hamster PrP protein (HaPrP) was expressed in E. coli and purified with HIS-tag affinity chromatograph. After being labelled with biotin, HaPrP was mixed with PrPSc preparation from scrapie strain 263K.</p><p><b>RESULTS</b>Protease-resistant bands were detected after four-day incubation.</p><p><b>CONCLUSION</b>The new conversion model provides a reliable, easily handling, and environment-friendly method for studies of prion and transmissible spongiform encephalopathies.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Peptídeo Hidrolases
/
Coloração e Rotulagem
/
Biotina
/
Proteínas Recombinantes
/
Sistema Livre de Células
/
Regulação da Expressão Gênica
/
Western Blotting
/
Cromatografia de Afinidade
/
Doenças Priônicas
Limite:
Animais
Idioma:
Inglês
Revista:
Biomedical and Environmental Sciences
Ano de publicação:
2006
Tipo de documento:
Artigo
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