In vitro anti-HIV activity of a Chinese fungus extract / 生物医学与环境科学（英文）

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the inhibitory effect of the mycelium extract from a Chinese fungus (M1) on HIV-1 and its mode of action.</p><p><b>METHODS</b>Several in vitro methods including time of action, time of addition and PCR were used to test the mode of action of M1.</p><p><b>RESULTS</b>M1 inhibited acute HIV infection in vitro and was effective when it was added 12 h after infection. PCR analysis of infected cells demonstrated that M1 delayed the appearance of late product of reverse transcription and HIV was blocked before its RNA expression.</p><p><b>CONCLUSION</b>The target of M1 is post-integration of proviral DNA.</p>