Metallothionein 1 isoform gene expression induced by cadmium in human peripheral blood lymphocytes / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 104-109, 2006.
Artigo
em Inglês
| WPRIM
| ID: wpr-229719
ABSTRACT
<p><b>OBJECTIVE</b>To study the gene expression of metallothionein 1 (MT-1) isoforms in human peripheral blood lymphocytes (HPBLs).</p><p><b>METHODS</b>The expression of mRNA representing the seven active MT-1 genes was determined in HPBLs by quantitative RT-PCR before and after exposure to cadmium.</p><p><b>RESULTS</b>Basal expressions of MT-1X, and MT-1A in HPBLs were similar to expression of housekeeping gene. In contrast, the basal gene expressions of MT-1H, 1F, 1E, and 1G were a little transcripts in human HPBLs. No signal was detected for MT-1B. There was a sex difference (P < 0.05). in basal gene expression of MT-1E. The levels of gene expression of MT-1A, 1E, 1F, 1G, 1H, and 1X increased, but the level of MT-1B did not increase after exposure to cadmium.</p><p><b>CONCLUSIONS</b>Gene expressions of MT-1G, MT-1H, MT-1F, and MT-1X in HPBLs can be used as a potential biomarker of cadmium exposure.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Cádmio
/
Linfócitos
/
Biomarcadores
/
Células Cultivadas
/
Regulação da Expressão Gênica
/
Primers do DNA
/
Isoformas de Proteínas
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Genética
Limite:
Adulto
/
Feminino
/
Humanos
/
Masculino
Idioma:
Inglês
Revista:
Biomedical and Environmental Sciences
Ano de publicação:
2006
Tipo de documento:
Artigo
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