Over-expressed genes detected by suppression subtractive hybridization in carcinoma derived from transformed 16HBE cells induced by BPDE / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 302-306, 2005.
Artigo
em Inglês
| WPRIM
| ID: wpr-229751
ABSTRACT
<p><b>OBJECTIVE</b>To screen the over differentially expressed genes in carcinoma induced by BPDE-transformed 16HBE cells (16HBE-C cells).</p><p><b>METHODS</b>The suppression subtractive hybridization (SSH) method was performed to profile differentially expressed genes between 16HBE-C cells and 16HBE cells. The cDNA fragments of differentially expressed genes were inserted into TA cloning vector and transformed competent E. coli strain. Positive clones were randomly picked up and identified by the colony PCR method. Dot blot was used to test the same source with the tester. The differentially expressed cDNA fragments were sequenced and compared with known genes and EST database in Genbank.</p><p><b>RESULTS</b>Eight known genes were over-expressed in 16HBE-C cells including eukaryotic translation elongation factor 1 alpha 1, HIF-1 responsive RTP801, ribosomal protein L10 (RPL10), ribosomal protein S29 (RPS29), mitochondrion related genes, and laminin receptor 1. Three differentially expressed cDNA fragments could not be matched to the known genes but to the EST database.</p><p><b>CONCLUSION</b>The SSH method can detect differentially expressed genes between 16HBE-C and 16HBE cells. BPDE-induced carcinogenesis may be related to alteration of at least eight known genes and three unknown genes. These expression data provide a clue to further cloning novel genes and studying functions in BPDE-induced carcinoma.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
7,8-Di-Hidro-7,8-Di-Hidroxibenzo(a)pireno 9,10-óxido
/
RNA Mensageiro
/
Linhagem Celular Transformada
/
Carcinógenos
/
Carcinoma
/
Regulação Neoplásica da Expressão Gênica
/
Linhagem Celular
/
Transformação Celular Neoplásica
/
Reação em Cadeia da Polimerase
Tipo de estudo:
Estudo prognóstico
Limite:
Humanos
Idioma:
Inglês
Revista:
Biomedical and Environmental Sciences
Ano de publicação:
2005
Tipo de documento:
Artigo
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