Responsive changes to MPTP in rats with DAT inhibited by antisense oligonucleotides / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 236-239, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-229781
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the inhibitory effect of antisense oligonucleotides (ODN) on dopamine transporter (DAT) in rats and observe the response of the rats to 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP).</p><p><b>METHODS</b>The cannula was implanted in the substantia nigra compacta under a rat stereotaxic device, through which drugs were used. The rats with successful operation were divided randomly into four groups, and received injection of antisense, sense, missense oligonucleotides and saline respectively, in the substantia nigra compacta of each rat via the cannula, followed by MPTP (30 mg/kg) injection. Behavior of the rats was observed and immunohistochemistry was carried out to check the expression of DAT and apoptosis of dopamine cell.</p><p><b>RESULTS</b>The expression of DAT (positive unit, PU) in the substantia nigra compacta in rats was 6.65+/- 1.67 in the antisense ODN group, 12.41+/- 2.46 in saline group, 11.45+/- 1.17 in sense ODN group, and 10.35+/- 2.89 in missense ODN group. The expression of DAT was lower in the antisense ODN group than that of the other three groups (P< 0.01). The rotation of the rats induced by apomorphine was slower than that of the other three groups(P< 0.05). The apoptotic cells (21.4+/- 5.6) in the antisense ODN group (200x ) were less than that of the other three groups (61.6+/- 19.7, 56.5+/- 16.3, 52.2+/- 12.5 respectively), (P< 0.01).</p><p><b>CONCLUSION</b>The expression of DAT can be inhibited effectively by the antisense ODN, and the response of the rats to the MPTP was reduced upon DAT inhibition.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Fisiologia
/
Imuno-Histoquímica
/
Apomorfina
/
1-Metil-4-Fenil-1,2,3,6-Tetra-Hidropiridina
/
Oligonucleotídeos Antissenso
/
Ratos Sprague-Dawley
/
Proteínas da Membrana Plasmática de Transporte de Dopamina
/
Genética
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Medical Genetics
Ano de publicação:
2008
Tipo de documento:
Artigo
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