Bioluminescent imaging monitoring of a anti-angiogenesis therapeutic gene vasostatin in tumor cell PC3 / 中国医学科学院学报

ABSTRACT

<p><b>OBJECTIVE</b>To generate a sensitive tool for noninvasive monitoring of a therapeutic gene vasostatin.</p><p><b>METHODS</b>We fused the bioluminescent reporter gene firefly luciferase to the therapeutic transgene vasostatin and ensured that these two proteins would not interrupt each other and kept their own natural character.</p><p><b>RESULTS</b>We therefore examined clones of PC3 cells stably expressing fusion gene and positive controlfluc with bioluminescence. In vivo imaging of PC3-Fluc subcutaneous tumors showed that the mean tumor bioluminescence increased in animals over several weeks.</p><p><b>CONCLUSION</b>Noninvasive monitoring facilitates the detection of gene expression in vivo and in vitro.</p>