Genetic diagnosis of polycystic kidney disease using single cell fluorescent polymerase chain reaction / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 283-286, 2007.
Artigo
em Chinês
| WPRIM
| ID: wpr-229986
ABSTRACT
<p><b>OBJECTIVE</b>To explore the feasibility of using single cell fluorescent polymerase chain reaction (PCR) in the preimplantation genetic diagnosis (PGD).</p><p><b>METHOD</b>Single buccal cell genetic analysis was performed with fluorescent PCR of linked microsatellite D16S423, followed by electrophoresis on ABI 3730.</p><p><b>RESULT</b>The amplification success rate, allele dropout rate, and diagnostic accuracy rate of the single cell fluorescent PCR were 93.3%, 10.7%, and 89.3%, respectively.</p><p><b>CONCLUSION</b>Single cell fluorescent PCR is a stable and reliable approach for the PGD.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Linhagem
/
Reação em Cadeia da Polimerase
/
Diagnóstico Pré-Implantação
/
Técnicas de Diagnóstico Molecular
/
Diagnóstico
/
Corantes Fluorescentes
/
Genética
/
Doenças Renais Policísticas
/
Métodos
Tipo de estudo:
Estudo diagnóstico
Limite:
Feminino
/
Humanos
/
Gravidez
Idioma:
Chinês
Revista:
Acta Academiae Medicinae Sinicae
Ano de publicação:
2007
Tipo de documento:
Artigo
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