Eimeria tenella cDNA library construction and expressed sequence tags analysis / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 1357-1362, 2007.
Artigo
em Chinês
| WPRIM
| ID: wpr-230686
ABSTRACT
In order to make a series of analyses on the gene expression of Eimeria tenella sensitive strain and anti-maduramycin strain at their different developmental stages, we constructed a mixed cDNA library with unsporulated oocyst, sporulated oocyst, sporozoite and merozoite from Eimeria tenella sensitive strain and antimaduramycin strain (induced by its sensitive strain)respectively. After sequencing reactions, the total 2806 high quality expressed sequence tags (ESTs) of 3' ends were derived from the cDNA library. Results of bioinformatics analysis of all EST data showed that EST sequences assembled 1424 tentative unique transcripts (TUTs) and the redundancy was 49.3%, and that about 83.6% TUTs could not be assigned for functional description. Among the remained annotated genes, infection related proteins and development related proteins such as MIC2 protein, BT1 family protein and some ribosomal protein expressed at high abundant level.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Biblioteca Gênica
/
Sitios de Sequências Rotuladas
/
Eimeria tenella
/
Etiquetas de Sequências Expressas
/
Genética
Idioma:
Chinês
Revista:
Journal of Biomedical Engineering
Ano de publicação:
2007
Tipo de documento:
Artigo
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