Investigation of canine mesenchymal stem cells differentiation to vascular endothelial cell in vitro / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 1348-1351, 2007.
Artigo
em Chinês
| WPRIM
| ID: wpr-230688
ABSTRACT
To induce endothelial cell, canine bone marrow-derived mesenchymal stem cells (MSCs) were separated from bone marrow by density gradient centrifugation. The isolated MSCs were induced to form endothelial-like cell in the presence of vascular endothelial growth factor (VEGF), endothelial growth factor (EGF) and so on. These results showed that the cells uniformly took on a cobblestone morphology under the light microscope, and cell nucleolus was in the middle of the cells. The cells displayed Weibel-Palade bodies under the transmission electron microscope. vWF, a specific marker of endothelial cell was positive in the cells. The above results demonstrate that MSCs may be differentiated into endothelial cells in vitro.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Células da Medula Óssea
/
Diferenciação Celular
/
Células Cultivadas
/
Fatores de Crescimento Endotelial
/
Técnicas de Cultura de Células
/
Biologia Celular
/
Células Endoteliais
/
Fator A de Crescimento do Endotélio Vascular
/
Células-Tronco Mesenquimais
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Biomedical Engineering
Ano de publicação:
2007
Tipo de documento:
Artigo
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