Isolation and purification of antimicrobial polypeptide HMGN2 from human lymph node and analysis of its distribution / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 842-846, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-230773
ABSTRACT
This study was conducted to isolate and purify antimicrobial polypeptides HMGN2 (high mobility group nucleosomal-binding domain2) from human lymph node, to detect the antimicrobial activity of HMGN2, and to determine the subcellular location of HMGN2 in human lymph node. The antimicrobial polypeptides were purified by the Reverse Phase HPLC and identified by Tricine-SDS-PAGE. The antimicrobial activity was detected by agar diffusion test. Mass spectrum and Western-blot analysis indicated the individual character of protein. HMGN2 was isolated and purified from human lymph node, and it showed antimicrobial potency against the pathogenic strain E. coli 54,080. The immunocytochemistry staining indicated that HMGN2 was present both in human lymph node cells' nucleus and cytoplasm. In conclusion, HMGN2 protein is of antimicrobial activity and it is probably involved in the defence of innate immunity in vivo.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Distribuição Tecidual
/
Química
/
Peptídeos Catiônicos Antimicrobianos
/
Proteína HMGN2
/
Escherichia coli
/
Linfonodos
/
Metabolismo
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Biomedical Engineering
Ano de publicação:
2010
Tipo de documento:
Artigo
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