Germplasm identification of Lonicerae Japonicae Flos by DNA melt curve analysis / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 4572-4578, 2016.
Artigo
em Chinês
| WPRIM
| ID: wpr-231018
ABSTRACT
To establish a new high resolution melting analytical method for identification of Lonicera japonica germplasm, the screening of 7 pairs of SSR (simple sequence repeats) primers, determining the suitable diagnostic primers by the differences of peak pattern and Tm was conducted. Then into the DNA template concentration, annealing temperature and the suitable range of cycle number were investigated. Combined with SIMCA-P software for data processing analysis, the results show that three main germplasm honeysuckle could be divided by four sets of primers. It provides methodology for improving L. japonica germplasm identification.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2016
Tipo de documento:
Artigo
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