Construction of a chimeric SEA-hPLAP-1 cDNA with gene splicing by overlap extension / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 412-414, 2003.
Artigo
em Chinês
| WPRIM
| ID: wpr-231035
ABSTRACT
<p><b>OBJECTIVE</b>To construct a chimeric SEA-hPLAP-1 cDNA with gene splicing by overlap extension.</p><p><b>METHODS</b>The SEA gene and a DNA fragment encoding the signal for GPI-anchor attachment of hPLAP -1 were amplified by PCR. The two amplified gene sequence was annealed to form a chimeric GPI- anchored SEA molecule with gene splicing by overlap extension. The resulting chimera was cloned in pGEM-T vector and verified by sequencing analysis.</p><p><b>RESULT</b>A chimeric SEA-hPLAP-1 cDNA was successfully constructed with gene splicing by overlap extension.</p><p><b>CONCLUSION</b>Gene splicing by overlap extension is a successful specific PCR technique for gene recombination.</p>
Texto completo:
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Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Proteínas Recombinantes de Fusão
/
Dados de Sequência Molecular
/
Sequência de Bases
/
Splicing de RNA
/
Reação em Cadeia da Polimerase
/
Fosfatase Alcalina
/
Enterotoxinas
/
Proteínas Ligadas por GPI
/
Genética
/
Isoenzimas
Idioma:
Chinês
Revista:
Journal of Zhejiang University. Medical sciences
Ano de publicação:
2003
Tipo de documento:
Artigo
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