Isolation, culturation and adipogenisis committed differentiation of adult human mesenchymal stem cell / 浙江大学学报·医学版

ABSTRACT

<p><b>OBJECTIVE</b>To isolate MSCs from adult human bone marrow cells and to induce them into adipocytes.</p><p><b>METHODS</b>MSCs were isolated from adult human bone marrow aspirated by Percoll and expanded in L-DMEM. The surface antigen of MSCs, CD14, CD34, CD45, CD44, VLA-1, HLA-DR and cell cycle were analysed on a FACScan flow cytometer. MSCs were cultured in adipogenisis inducing medium including insulin, 1-methyl-3-isobutylxanthine, indomethine and dexamethasone for 7 days and stained with Oil Red O.</p><p><b>RESULTS</b>MSCs grew as adherent cells and expanded more than 10 passages. They were positive for CD44 and negative for CD14, CD34, CD45, HLA-DR. The expression of VLA-1 was weak. After 7 days of adipocyte inducing, about 85%of the cells displayed accumulation of lipid vacuoles, as detected by Red Oil O.</p><p><b>CONCLUSION</b>MSCs isolated and cultured from adult human bone marrow can be induced to adipogenisis committed differentiation.</p>