Improvement of cardiomyocyte isolation for different purposes / 浙江大学学报·医学版

ABSTRACT

<p><b>OBJECTIVE</b>To improve the methods of isolating single ventricular myocytes for purposes of different experiments.</p><p><b>METHODS</b>The cardiac ventricular myocytes were isolated enzymatically by Langendorff perfusion technique at constant flow rate. The patch clamp whole-cell recording, a spectrofluorometric method and a video tracking system were used to verify the basic electrophysiological properties, intracellular calcium transient and cell contraction of the single myocytes.</p><p><b>RESULTS</b>The resting membrance potential of the myocyte was (-74.06+/-4.54)mV. The whole-cell sodium, potassium and calcium currents, intracellular calcium transients and contractile properties were stable and typical.</p><p><b>CONCLUSION</b>The myocytes obtained by adjusting perfusion parameters are well suited for electrophysiological recording, intracellular calcium and single cell contraction measurements.</p>