Sequencing and analyses of the adenovirus polymerase gene in fecal samples of captively bred Rhesus macaques / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 268-270, 2011.
Artigo
em Chinês
| WPRIM
| ID: wpr-231134
ABSTRACT
<p><b>OBJECTIVE</b>In an attempt to study the moleculr characterization and epidemiology of simian adenoviruses in nonhuman primate (NHP) populations.</p><p><b>METHODS</b>We examined a colony of captively bred rhesus macaques (Macaca mulatta) in China for the presence of adenoviral DNA in stool samples. This was done by using the PCR method that targeted the adenovirus polymerase gene, and the PCR positive fragments were cloned for sequencing and phylogenetic analyses.</p><p><b>RESULTS</b>Among the 57 animals analyzed, fecal samples from 12 animals were positive for the presence of adenoviral DNA. The results suggested that the viral DNA clones were primarily segregated into two large groups SAdV-6 (2 non-redundant sequences) and SAdV-7 (9 non-redundant sequences). In addition, there were three clones with more similarity to SAdV-1, SAdV-3 and HAdV-52 respectively.</p><p><b>CONCLUSION</b>Our data confirmed the prevalence of adenoviral DNA in the feces of NHPs and revealed the adenoviruses in the gastrointestinal tract of the study animals. heterogeneity and phylogenetics of the adenoviruses in the gastrointestinal tract of the study animals.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Filogenia
/
Proteínas Virais
/
Virologia
/
Dados de Sequência Molecular
/
Adenoviridae
/
Classificação
/
Análise de Sequência de DNA
/
DNA Polimerase Dirigida por DNA
/
Fezes
/
Genética
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Experimental and Clinical Virology
Ano de publicação:
2011
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS