Cloning, expression and bio-activity assay of chimeric fusion protein sTNFRII-IgG Fc / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 178-181, 2002.
Artigo
em Chinês
| WPRIM
| ID: wpr-231355
ABSTRACT
Tumor necrosis factor (TNF) is a key cytokine in immunology system and is related to many human diseases. In order to inhibit the activity of TNF, cDNA coding for soluble TNF receptor II (sTNFRII) and human IgG Fc were linked using a flexible hinge. This gene was expressed in E. coli as a chimeric protein and purified by metal chelate chromatography. The results show that the fusion protein exists in the physiological form as a dimer, has the ability to bind with TNF and inhibits the cytotoxicity of TNF on L929 cells. Contrasting to monomer sTNFRII, the chimeric protein has an improved bioactivity, and displays potential prospects for research and application.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Recombinação Genética
/
Proteínas Recombinantes de Fusão
/
Imunoglobulina G
/
Fragmentos Fc das Imunoglobulinas
/
Engenharia Genética
/
Antígenos CD
/
Expressão Gênica
/
Western Blotting
/
Cromatografia Líquida
/
Fator de Necrose Tumoral alfa
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2002
Tipo de documento:
Artigo
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