Extraction of the salt-tolerant cDNA in mangrove Avicennia marina by mRNA differential display / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 51-54, 2002.
Artigo
em Chinês
| WPRIM
| ID: wpr-231374
ABSTRACT
RNAs of the leaves in Avicennia marina, which cultured in 50@1000 and 0@1000 salinity condition respectively, were isolated for mRNA differential display analysis. Screened by OligodT12 GC and eight 10-oligonucleotide arbitrary primers, differential cDNA fragments-csrg1(600 bp), csrg2(550 bp), csrg3(480 bp), only appeared in the leaves of Avicennia marina cultured in 50@1000 salinity condition. After detected by RNA dot hybridization, only csrg1 appeared the difference between the RNAs of the leaves in Avicennia marina cultured in 50@1000 and 0@1000 salinity condition respectively, and csrg1 was confirmed as the salt-tolerant cDNA. csrg1 was cloned and sequenced. After searching in Genbank, there were no similar sequences reported.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Fisiologia
/
Sais
/
Sequência de Bases
/
Clonagem Molecular
/
Análise de Sequência de DNA
/
DNA Complementar
/
Folhas de Planta
/
Regulação da Expressão Gênica de Plantas
/
DNA de Plantas
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2002
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS