Cloning and expression of Buthus martensii Karsch scorpion toxin gene (BmK IT3) in Escherichia coli / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 106-108, 2002.
Artigo
em Chinês
| WPRIM
| ID: wpr-231387
ABSTRACT
According to the reported sequence of Buthus martensii Karsch scorpion toxin gene (BmK IT3), we synthesized two primers, which were complementary in a region. By the means of PCR, we got the gene. The gene was fused in expression vector pET-28a, which gave rise to a recombinant plasmid pET(IT3R). Then it was transformed into E. coli BL21 (DE3). With IPTG induction, the gene was efficiently expressed. And the fusion product was soluble.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Venenos de Escorpião
/
Escorpiões
/
Proteínas Recombinantes
/
Expressão Gênica
/
Química
/
Clonagem Molecular
/
Escherichia coli
/
Genética
/
Isopropiltiogalactosídeo
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2002
Tipo de documento:
Artigo
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