Effect of bone morphogenetic protein-7 on aristolochic acid induced renal tubular epithelial cells transdifferentiation / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
;
(12): 157-160, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-231567
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effect of bone morphogenetic protein-7 (BMP-7) on aristolchic acid induced renal tubular epithelial cell trans-differentiation to look for new therapeutic approach for aristolchic acid nephropathy (AAN).</p><p><b>METHODS</b>In vitro cultured human proximal renal tubular epithelial cell line HK-2 cells were treated with different concentrations of BMP-7 (75 ng/mL, 150 ng/mL and 300 ng/mL) after trans-differentiation of the cells was induced by AA (10 microg/mL). Levels of alpha-SMA mRNA and protein expressions were detected by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and Western blotting respectively.</p><p><b>RESULTS</b>BMP-7 reversed the AA inducing alpha-SMA expressions in HK-2 cells in a dose-dependent manner.</p><p><b>CONCLUSION</b>BMP-7 can inhibit the trans-differentiation of human renal tubular epithelial cell induced by AA, thereby might be a new potential drug for AAN prevention and treatment.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Linhagem Celular
/
Actinas
/
Biologia Celular
/
Ácidos Aristolóquicos
/
Células Epiteliais
/
Transdiferenciação Celular
/
Proteína Morfogenética Óssea 7
/
Túbulos Renais Proximais
/
Metabolismo
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Integrated Traditional and Western Medicine
Ano de publicação:
2010
Tipo de documento:
Artigo
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