Enhanced action of a BTB/POZ domain protein on the expression of hsp90alpha gene in heat shock / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 392-395, 2004.
Artigo
em Chinês
| WPRIM
| ID: wpr-231922
ABSTRACT
<p><b>OBJECTIVE</b>To study the role of a BTB/POZ domain protein in the expression of hsp90alpha gene.</p><p><b>METHODS</b>The eukaryotic expression plasmids of sense- and antisense-GAGA related protein (GRP) or empty vector were transfected into Jurkat cells with pREP4 episomal vector plasmids carrying the hsp90alpha promoter sequence from -1756 to +37 and control plasmids pMCAT. Total RNA was extracted. The relative promoter activity of hsp90alpha-CAT reporter gene was determined by competitive RT-PCR assay.</p><p><b>RESULTS</b>GRP markly increased the relative promoter activity of hsp90alpha-CAT reporter gene during heat shock.</p><p><b>CONCLUSION</b>GRP may promote the expression of hsp90alpha gene by participating in chromatin remolding.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Proteínas Repressoras
/
Fatores de Transcrição
/
DNA
/
Química
/
Regulação da Expressão Gênica
/
Clonagem Molecular
/
DNA Complementar
/
Proteínas de Homeodomínio
/
Proteínas de Choque Térmico HSP90
/
Resposta ao Choque Térmico
Limite:
Animais
/
Humanos
Idioma:
Chinês
Revista:
Acta Academiae Medicinae Sinicae
Ano de publicação:
2004
Tipo de documento:
Artigo
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