Drug screening model acting on out-membrane protein OprM in pseudomonas aeruginosa efflux pump system / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 359-363, 2004.
Artigo
em Chinês
| WPRIM
| ID: wpr-231928
ABSTRACT
<p><b>OBJECTIVE</b>To establish an efflux pump inhibitor screening model with the out-membrane protein OprM in Pseudomonas aeruginosa efflux pump system as the target point.</p><p><b>METHODS</b>Efflux pump out-membrane protein gene oprM was obtained from standard Pseudomonas aeruginosa PA01 strain. Expression of OprM protein was induced in E. coli strain HS151 with T-easy vector as the cloning vector, and pMMB67EH as the expression vector. In order to evaluate the function of OprM protein, we measured intracellular tetracycline concentrations with liquid scintillation counter, measured the diameters of bacteriostatic circles with paper disc, and then established a screening model accordingly.</p><p><b>RESULTS</b>OprM protein was highly expressed. Using Pseudomonas aeruginosa as the main detecting bacteria, we established a drug screening model acting on OprM. A total of 1 600 microbial fermentation samples were screened with this model, among which 56 positive strains were found, with a positive rate of 3.5%.</p><p><b>CONCLUSION</b>OprM plays an important role in drug efflux. The established model has good specificity and maneuverability.</p>
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Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Proteínas de Membrana Transportadoras
/
Farmacologia
/
Plasmídeos
/
Pseudomonas aeruginosa
/
Proteínas da Membrana Bacteriana Externa
/
Proteínas de Bactérias
/
Resistência Microbiana a Medicamentos
/
Resistência a Múltiplos Medicamentos
/
Avaliação Pré-Clínica de Medicamentos
/
Escherichia coli
Tipo de estudo:
Estudo diagnóstico
/
Estudo de rastreamento
Limite:
Humanos
Idioma:
Chinês
Revista:
Acta Academiae Medicinae Sinicae
Ano de publicação:
2004
Tipo de documento:
Artigo
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