Effects of two different cryoprotectants on the motility of post-thaw human sperm / 中华男科学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To compare the effects of the cryoprotectant containing glucose and that containing sucrose on the motility of post-thaw human sperm.</p><p><b>METHODS</b>The cryoprotectant containing glucose and that containing sucrose were applied to 50 semen samples and the motility of the post-thaw human sperm was compared before and after cryopreservation and between the study groups.</p><p><b>RESULTS</b>The forward motility and total motility of the sperm were (58.4 +/- 5.7)% and (63.4 +/- 6.1)% before cryopreservation, (43.8 +/- 7.6)% and (48.4 +/- 7.6)% after thawing with the cryoprotectant containing glucose, and(42.6 +/- 8.9)% and (48.0 +/- 8.5)% after thawing with the cryoprotectant containing sucrose. Decreased sperm motility was observed after cryopreservation, with statistic significance (P < 0.01). There was no significant difference in the forward and total motility of the post-thaw sperm between the two cryoprotectants.</p><p><b>CONCLUSION</b>Cryopreservation inflicts obvious damage on sperm. Sucrose is a feasible sperm cryoprotectant.</p>