Construction and application of a multiplex PCR system for genotyping of human red blood cell antigens / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 547-552, 2012.
Artigo
em Chinês
| WPRIM
| ID: wpr-232259
ABSTRACT
<p><b>OBJECTIVE</b>A reliable method for genotyping blood group antigens Dib, k, Jsb1910 and Jsb2019 was developed. Through screening for rare blood types, the National Rare Blood Bank of China may be enriched.</p><p><b>METHODS</b>The controls for allele detection of blood groups Dib, k, Jsb1910 and Jsb2019 were prepared via polymerase chain reaction (PCR)-mediated gene site-directed mutagenesis (SDM) technique. Sequence-specific primers were designed according to known single nucleotide polymorphism (SNP) sites of alleles of blood groups antigens Dib, k, Jsb1910 and Jsb2019, a multiplex PCR system was developed by optimizing PCR reaction system. And 4190 random healthy donors samples were screened for the blood group antigens.</p><p><b>RESULTS</b>Using SDM technique, controls for alleles in blood group Dib, k, Jsb1910 and Jsb2019 were successfully generated. And a multiplex PCR system for genotyping above blood groups was developed. After verification, the system has performed with good stability and reproducibility. Two Di (b-) samples have been discovered from 4190 samples, no k- and Js(b-) sample was found.</p><p><b>CONCLUSION</b>Multiplex PCR features rapid detection, high throughput and low cost, and can be used for screening for donors of rare blood types. Information of donors may be registered in a database, which in turn can help those with rare blood types or require long-term blood transfusion to obtain matched blood, thereby reduce the adverse reactions of blood transfusion.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Antígenos de Grupos Sanguíneos
/
Mutagênese Sítio-Dirigida
/
Polimorfismo de Nucleotídeo Único
/
Alergia e Imunologia
/
Eritrócitos
/
Técnicas de Genotipagem
/
Reação em Cadeia da Polimerase Multiplex
/
Genética
/
Métodos
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Medical Genetics
Ano de publicação:
2012
Tipo de documento:
Artigo
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