Study on the kinesis of cellular immunity in adults vaccinated with recombinant hepatitis B vaccine / 中华流行病学杂志
Chinese Journal of Epidemiology
;
(12): 326-330, 2007.
Artigo
em Chinês
| WPRIM
| ID: wpr-232311
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the kinesis of cellular immunity in adults who were vaccinated with yeast recombinant hepatitis B(rHB) vaccine and the correlation between cellular and humoral immune responses induced by the vaccine.</p><p><b>METHODS</b>Eight adults were vaccinated with rHB vaccine according to 0, 1,2 month schedule. The peripheral blood mononuclear cells(PBMCs) were collected at the 3, 8, 21, 34 and 65 days after the first dose. The high purity of CD4+ and CD8+ T cells obtained by sorting from PBMCs were restimulated with recombinant hepatitis B surface antigens (rHBsAg) or peptides. The spot forming cell (SFC) of IFN-gamma, IL-2 and IL-4 of CD4+ and CD8+ T cells were detected by enzyme-linked immunospot (ELISPOT).</p><p><b>RESULTS</b>The characteristics of IFN-gamma, IL-2 and IL-4 of CD4+ and CD8+ T cells appeared different after immunization with rHB vaccine. IFN-gamma of CD8+ and CD4+ T cells could be detected early with stable SFC, while the IL-2 and IL-4 of CD4+ T cells appeared late but increased after the second and third dose of vaccination. The positive rate of IL-4 of CD4+ T cells were significantly correlated with the positive rate of anti-HBs, while the SFCs of IL-4 and IL-2 of CD4+ T cells were also significantly related to the titers of anti-FIBs.</p><p><b>CONCLUSION</b>IFN-gamma could be detected early after rHB vaccination in adults, and the positive rates of IL-4 and IL-2 were correlated with that of anti-HBs.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Sangue
/
Vacinas Sintéticas
/
Linfócitos T CD4-Positivos
/
Esquemas de Imunização
/
Interleucina-4
/
Interferon gama
/
Interleucina-2
/
Vacinas contra Hepatite B
/
Linfócitos T CD8-Positivos
/
Alergia e Imunologia
Limite:
Adulto
/
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Epidemiology
Ano de publicação:
2007
Tipo de documento:
Artigo
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